muscle growth medium mgm Search Results


rat msc growth medium kit  (Cell Applications Inc)
96
Cell Applications Inc rat msc growth medium kit
A,D) Brightfield photomicrographs of REFs transfected with siCntrl, siPrrx1 & siSnai2, (Rows 1,2 and 3) that were incubated in <t>MSC</t> media or Rat ESC media for 14 days. IF photomicrographs assess expression of MSC TF Myc (Red) and ESC TFs (Sox2 and Nanog in Red) and DAPI (Blue) in all three groups (Scale bar, 10µm). D) (Column 4) Brightfield photomicrographs of free-floating embryoid bodies on day 8 of suspension culture in differentiation media <t>following</t> <t>transfection</t> of REFs with siCntrl, siPrrx1 & siSnai2 and incubation in Rat ESC media for 14 days. B) MSC activity was ascertained using Alkaline Phosphatase Assay. C,E) q-RTPCR analysis to assess increase in relative expression of Myc, Klf4, Sox2 and Nanog compared to housekeeping gene (B2m) in siPrrx1 and siSnai2 treated REFs when incubated in MSC and ESC media respectively for 14 days. F, G and H) IF Confocal microscopy images assess expression of Ectodermal TFs Sox2 (green) and Otx-2 (red), Mesodermal TFs Brachyury (green) and Hand1 (red), and Endodermal TFs Gata-4 (green) and Sox17 (red) of embryoid bodies at Day 8 in the siSnai2 group, nuclear counterstain DAPI (blue).
Rat Msc Growth Medium Kit, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/muscle+growth+medium+mgm/Major+Media/bio_rxiv__2020__03__19__999433-132-14-19
Average 96 stars, based on 1 article reviews
rat msc growth medium kit - by Bioz Stars, 2026-09
96/100 stars
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smgmtm- 2 smooth muscle cell growth medium  (Lonza)
N/A
Culture system containing SmBMTM Basal Medium (CC-3181) and SmGMTM-2 SingleQuotsTM supplements (CC-4149) required for growth of Smooth Muscle Cells
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smgmtm- 2 smooth muscle cell growth medium-2 singlequotstm supplements  (Lonza)
N/A
SmGMTM-2 Smooth Muscle SingleQuotsTM Kit, Supplements and growth factors required for culture of Human Smooth Muscle Cells (basal media sold separately).
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A,D) Brightfield photomicrographs of REFs transfected with siCntrl, siPrrx1 & siSnai2, (Rows 1,2 and 3) that were incubated in MSC media or Rat ESC media for 14 days. IF photomicrographs assess expression of MSC TF Myc (Red) and ESC TFs (Sox2 and Nanog in Red) and DAPI (Blue) in all three groups (Scale bar, 10µm). D) (Column 4) Brightfield photomicrographs of free-floating embryoid bodies on day 8 of suspension culture in differentiation media following transfection of REFs with siCntrl, siPrrx1 & siSnai2 and incubation in Rat ESC media for 14 days. B) MSC activity was ascertained using Alkaline Phosphatase Assay. C,E) q-RTPCR analysis to assess increase in relative expression of Myc, Klf4, Sox2 and Nanog compared to housekeeping gene (B2m) in siPrrx1 and siSnai2 treated REFs when incubated in MSC and ESC media respectively for 14 days. F, G and H) IF Confocal microscopy images assess expression of Ectodermal TFs Sox2 (green) and Otx-2 (red), Mesodermal TFs Brachyury (green) and Hand1 (red), and Endodermal TFs Gata-4 (green) and Sox17 (red) of embryoid bodies at Day 8 in the siSnai2 group, nuclear counterstain DAPI (blue).

Journal: bioRxiv

Article Title: Gatekeeper transcription factors regulate switch between lineage preservation and cell plasticity

doi: 10.1101/2020.03.19.999433

Figure Lengend Snippet: A,D) Brightfield photomicrographs of REFs transfected with siCntrl, siPrrx1 & siSnai2, (Rows 1,2 and 3) that were incubated in MSC media or Rat ESC media for 14 days. IF photomicrographs assess expression of MSC TF Myc (Red) and ESC TFs (Sox2 and Nanog in Red) and DAPI (Blue) in all three groups (Scale bar, 10µm). D) (Column 4) Brightfield photomicrographs of free-floating embryoid bodies on day 8 of suspension culture in differentiation media following transfection of REFs with siCntrl, siPrrx1 & siSnai2 and incubation in Rat ESC media for 14 days. B) MSC activity was ascertained using Alkaline Phosphatase Assay. C,E) q-RTPCR analysis to assess increase in relative expression of Myc, Klf4, Sox2 and Nanog compared to housekeeping gene (B2m) in siPrrx1 and siSnai2 treated REFs when incubated in MSC and ESC media respectively for 14 days. F, G and H) IF Confocal microscopy images assess expression of Ectodermal TFs Sox2 (green) and Otx-2 (red), Mesodermal TFs Brachyury (green) and Hand1 (red), and Endodermal TFs Gata-4 (green) and Sox17 (red) of embryoid bodies at Day 8 in the siSnai2 group, nuclear counterstain DAPI (blue).

Article Snippet: Seven days post initial siRNA transfection, transfected cells were placed in Rat MSC medium (Rat MSC growth medium kit, Cell Applications, Inc.) and incubated for 14 days in incubator (37°C, 5% CO2).

Techniques: Transfection, Incubation, Expressing, Suspension, Activity Assay, ALP Assay, Reverse Transcription Polymerase Chain Reaction, Confocal Microscopy